Kanagawa Accademy of Science and Technology
Project for the establishment of innovative platelet manufacturing technology and its clinical application
Aiming for the establishment of a novel system for the stable supply of ex vivo produced platelets
 

 Based on a novel regenerative medicine/cell culture technology developed by the project leader, we seek to establish a technology for the manufacture of novel, safe and stable platelets and to develop an assessment system for the function of produced platelets.

Project Leader: Ms.Yumiko Matsubara
Investigation period: 2014-2018
Laboratory members:
・Project Leader
・Full time Researcher
・Temporary Researcher
・Research assistant
・Office Personnel

Research outline

  In this research, we aim to establish a stable platelet supply system where platelets are prepared from previously obtained and stored one’s own fat cells, and used for transfusion when required. If the system becomes available, major problems (will be discussed later) in platelet transfusion such as shortage, infection and loss of its effectiveness due to repeated transfusions will be solved.

  Platelets are unique in having an ability to stop bleeding, and therefore, a severe decrease in platelet counts during chemotherapy may cause uncontrollable bleeding and death. Platelet transfusion is an established treatment for thrombopenia; however, its supply is unstable as it completely depends on voluntary blood donations and it has a short shelf life of 4 days. Thus, in this super aging-society with ever-increasing cancer patients, the demand of platelets is going to exceed its supply in the near future.

  This research aims to develop a system to produce a large amount of safe and high-quality platelets from a small amount of subcutaneous preadipocytes. Furthermore, in order to assess the safety and functions of the produced platelets such as a hemostatic ability, we conduct in vitro studies as well as in vivo studies using immunodeficient mice. Moreover, we will optimize a system for introduction of various large-scale bioreactors and establish storage systems suitable to maintain the function of the produced platelets.

 

Research content

(1)Protocol optimization for platelet production

Aiming to scale-up the platelet production protocol previously established, we seek to optimize the protocol, conditions and such including a large-scale cultivation of megakaryocytes which release platelets.
 

(2)Assessment of the functions of produced platelets, and development of associated assay systems

In vitro functional assessment of produced platelets
  In vitro capabilities such as granule release, platelet aggregation, and thrombus formation.
② Functional assessment and safety studies of produced platelets
  Study of wound healing effect of produced human platelets using a wounded NOG(immunodeficient)mouse.
 

(3)In vivo Functional assessment and safety studies of produced platelets

In vivo functional assessment of produced platelets using a thrombocytopenic NOG(immunodeficient)mouse.
 

(4)Establishment of a large scale cultivation method and a preservation technology to maintain the function of produced platelets for the clinical application

Establishment of a protocol for the introduction of a large- scale bioreactor